The UCHL1 Inhibitor Screening Kit utilizes the ability of active UCH-L1 deubiquitinase to cleave a synthetic protein substrate to release the free fluorophore, which can be easily quantified (Ex/Em = 350/440 nm) using a fluorescence microplate reader. Small molecule inhibitors can either reduce or abolish this activity. This inhibitor screening kit thus allows rapid and reliable determination of the inhibitory effects of various compounds on UCH-L1 deubiquitinase and should be used to screen for novel inhibitors.
Size
100 assays
Concentration
n/a
Applications
Screening potential inhibitors/ligands of UCH-L1Characterizing/studying UCH-L1 inhibitors