Short-chain fatty acids (SCFAs) are a sub-group of saturated aliphatic fatty acids contain 2-5 carbon (C) atoms, while acetate (C2), propionate (C3), and butyrate (C4) are the most abundant. They are the end products of fermentation of the dietary fibers by beneficial large intestinal bacteria. Creative Proteomics has established sensitive, reliable, and accurate GC-MS method for quantification of short chain fatty acids.