The abnormal cell migration and invasion can lead to disease progression including cancer, atheroscelerosis and arthritis. The research of cell invasion has great significance and the invasion assay can facilitate the mechanism research of cell invasion. The invasion assay is widely used in the studies of cell behaviors and assessment of the metastatic potential of tumor cells. There are many methods to research cell invasion. Among all of the assays used for the research of cell invasion, transwell invasion assay is one of the most widely used and convenient technique to research cell invasion.