Protein Fragment AnalysisReversed-phase Chromatography (RP)
Description
In reversed-phase HPLC (RP-HPLC), stationary phase is non-polar and mobile phase has a moderately polar characteristic. Silica is most common stationary phase for RP-HPLC. Acetonitrile and methanol are usually used as mobile phases. RP-HPLC operates based on the hydrophobic interactions and allows the measurement of these interactive forces. This technique is often and favored used for both peptide and protein separations due to its superior resolution, good repeatability and the compatibility of eluents with MS analysis. The pharmaceutical industry regularly employs RP-HPLC to qualify drugs before their release.