Immunofluorescence, Immunoprecipitation, Western Blot
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Immunogen
purified adult chicken kidney (Na+, K+)-ATPase beta-subunit: Total protein