This Mouse/Rat Urocortin 3 ELISA Assay kit for determination of mouse/rat urocortin 3 in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse/rat urocortin 3 with biotin-avidin affinity system. To the wells of plate coated with rabbit anti mouse/rat urocortin 3 antibody, standards or samples and labeled Antigens (biotinylated Antigens) are added for competitive immunoreaction. After incubation and plate washing, horseradish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-labeled Antigens-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-This Mouse/Rat Urocortin 3 ELISA Assay kit for determination of mouse/rat urocortin 3 in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse/rat urocortin 3 with biotin-avidin affinity system. To the wells of plate coated with rabbit anti mouse/rat urocortin 3 antibody, standards or samples and labeled Antigens (biotinylated Antigens) are added for competitive immunoreaction. After incubation and plate washing, horseradish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-labeled Antigens-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse/rat urocortin 3 is calculated. Sample Types: mouse/rat serum, plasma. Range: 0.41 - 100 ng/mL. Time: 21 hours ? Urocortin 3 (Ucn3) or stresscopin (SCP) is a new member of the corticotropin-releasing factor (CRF) peptide family identified in the mouse and human. The CRF family of neuropeptides includes mammalian peptides CRF, urocortin 1(Ucn1) and urocortin 2 (Ucn2) or stress-related peptide (SRP), as well as piscine urotensin 1 and frog sauvagine. Mouse and human Ucn3 share 90% identity in the 38-aa putative mature peptide.