The Eagle Biosciences Mouse TNF alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-αpresent is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF-α is added to the wells and binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 15.625 - 500 pg/ml. Sensitivity: 7 pg/ml. Time: 3.0 hours ? The Eagle Biosciences Mouse TNF-α (TNF-α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 12 (TNF-α) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Mouse TNF Alpha (TNF-α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.