The Eagle Biosciences MutaPLATE? HLA-DQ2+8 (TM) real time PCR Kit contains three allele specific primers and TaqMan probes, labeled with FAM, for the detection of the HLA-DQ2 & DQ8 alleles. The TaqMan probes bind to the amplificated target-DNA. Due to this, a fluorescence signal is generated and detected by the optical unit of the real time PCR instrument (e. g. Light Cycler?, Roche). Positive samples are identified due to the increase in fluorescence on the FAM (530 nm) channel during the PCR. An internal amplification control (IC), labeled with Yakima Yellow (560 nm), is also amplified with every sample. For a valid result, there must be a successful amplification of the IC on the YAK channel. Sample Types: DNA (e.g.whole blood, cheek swab). ? Celiac disease (CD) / gluten intolerance (GI) is one of the most often chronic gastrointestinal diseases. The disease is characterised intolerance for gliadin fractions in wheat or analogous proteins in other cereals. The intake of gluten with food causes by patients chronic but reversible damages of the gastrointestinal mucous membrane which finally manifests histological in villious atrophy of the small intestine. CD/ GI is genetically strong associated with the alleles DQA1*05 (=0501)/ DQB1*02 (=0201 and 0202) and DQB1*0302.