The Eagle Biosciences MutaPLATE? Laktase (TAQ-Man) real time PCR Kit contains specific primers and additional material for the detection of the T/C (-13910) polymorphism in the regulatory region of the lactase phlorizin hydrolase gene. The variable area of the regulatory region from lactase gene is amplified by PCR using genomic DNA-template. The specific primers used in the kit flank the variable area of lactase gene (LCT) and generate an amplificate of 300 bp using Taq-Man technology. The standard PCR contains additionally two sequence specific oligonucleotides marked with fluorescence dye (TaqMan probes). Both probes bind at the amplificated target-DNA which includes the single nucleotide polymorphism (SNP). Due to this, a fluorescence signal is generated and detected by the optical unit of the used real time PCR instrument. The TaqMan probe for the C-allele (mutation) is marked with FAM (510nm, green) and the TaqMan probe for the T-allele (wildtype) is marked with YAK (555 nm, yellow). Sample Types: DNA (e.g.whole blood, cheek swab). ? The Eagle Biosciences MutaPLATE? Lactase (TAQ-Man) real time PCR kit is a molecular biological test for analysis of the -13910 T/C polymorphism in the regulatory region of lactase phlorizin hydrolase gene (LPH) in open real time PCR systems (e. g. RotorGene, SmartCycler, Light Cycler, ABI, Stratagene, Amplifa). The clinical relevant C/C - genotype causes primary lactase deficiency often leading to (genetic) lactose intolerance. The Eagle Biosciences MutaPLATE? Lactase (TAQ-Man) is for research use only and is not intended for diagnostic or therapeutic procedures.