This Rat Prorenin / Renin ELISA Kit uses a capture antibody coated to the 96-well plate to bind Rat Prorenin and Renin. A detection antibody conjugated to biotin is then applied. After washing, avidin conjugated to horseradish peroxidase (HRP) is applied. TMB substrate is added for color development which is proportional to the total concentration of prorenin and rennin in the sample. Sample concentrations can be determined by comparing OD values to the standard curve. Sample Types: biological fluids. Range: 0.1 - 100 ng/mL. Sensitivity: 0.1 ng/mL. Time: 1.5 hours ? Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects