The Eagle Biosciences Sclerostin ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of sclerostin in serum or heparin plasma. The Eagle Biosciences Sclerostin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. Sample Types: human serum, heparin plasma. Range: 0 to 240 pmol/l. Sensitivity: LOD: (0 pmol/l + 3 SD): 3.2 pmol/l; LLOQ: 24 hours ? Canonical Wnt-signalling plays an important role in the regulation of bone homeostasis by promoting the development of osteoblasts. Negative regulators of the Wnt pathway are important new therapeutic targets for the treatment of diseases with enhanced bone resorption. One of these molecules is Sclerostin, a 22.5 kD secreted glycoprotein, which acts by binding to the Wnt-coreceptor LRP5 thus preventing the binding of Wnt molecules. Sclerostin is nearly exclusively produced in osteocytes. Therefore it is considered a clinical marker which provides highest bone specificity.