Pretreated EDTA whole blood samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus.The addition of Vitamin B2 in either standards [STD] or samples gives a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37°C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 - 630nm (alternatively at 540 - 550nm) in an ELISA-reader and a standard curve is generated from the dilution series.The amount of Vitamin B2 is directly proportional to the turbidity. Sample Types: whole blood. Range: 5 - 60 μg/L. Sensitivity: 5 μg/L. Time: 48 hours ? The Eagle Biosciences Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood.The test kit contains all required reagents, e.g.standard,medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.