The Eagle Biosciences Cetuximab ELISA is based on Cetuximab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone IG-FB7D9). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-FB7D9 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Cetuximab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Cetuximab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Cetuximab to the solid phase, pre-coated with IG-FB7D9, is inhibited by recombinant human epidermal growth factor receptor (rh-EGFR) protein in a concentration dependent manner. Therefore, the ImmunoGuide Cetuximab ELISA (mAb-Based) measures the free form of Cetuximab. Sample Types: serum, plasma. Range: 6 - 200 ng/mL. Sensitivity: 2 ng/ml. Time: 2 hours ? The drug Cetuximab (trade name Erbitux?) is a human-mouse chimeric immunoglobulin (Ig) G1? monoclonal antibody (mAb), selectively directed against the epidermal growth factor receptor (EGFR), also known as HER1 or ErbB1. Since its approval by Food and Drug Administration for cancer treatment in 2004, Cetuximab became widely used in the treatment of colorectal and head and neck cancers. The ImmunoGuide Cetuximab ELISA (mAb-based) kit can be efficiently used for measuring free Cetuximab levels in serum and plasma.