The Eagle Biosciences ELISA for Chemerin is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The Chemerin in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-Chemerin-Antibody binds in turn to the immobilised Chemerin. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the Chemerin-level of the samples. Sample Types: serum, plasma. Range: 25 - 600 pg/mL. Sensitivity: 5 pg/ml. Time: 2.5 hours ? Chemerin, also known as tazarotene-induced gene 2 (TIG2) or retinoic acid receptor responder 2 (RARRES2), is synthesized as precursor protein of 163 amino acids including a N-terminal signal peptide of 20 amino acids, which is chipped of enduring secretion. The inactive, circulation proform of Chemerin contains six cystein residues and thus three intramolecular disulfide bridges are suggested (1).