The Eagle Biosciences Human DKK-1 ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of Human DKK-1 in serum. The Eagle Biosciences Human DKK-1 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. Sample Types: human serum or plasma (citrate, EDTA or heparin). Range: 0 to 160 pmol/l. Sensitivity: LOD: 1.7 pmol/l (0 pmol/l + 3 SD) , LLOQ: 1.25 pmol/l. Time: 3.5 hours ? Dickkopf-1(DKK-1) is a 28.67 kDa secreted protein that acts as soluble inhibitor of the WNT signalling pathway. This pathway contains lipid-modified glycoproteins that activate cell surface receptor-mediated signal transduction to regulate cell activities like: cell fate, proliferation, migration, polarity and gene expression. DKK-1 regulates developmental processes of all kinds. Thus, DKK-1 is also involved in the regulation of bone metabolism as it effects osteoblast differentiation and in regulation of tumourgenetic activity.