Dopamine & Noradrenaline High Sensitive ELISA Assay kit
Description
The competitive Dopamine & Noradrenaline High Sensitive ELISA Assay kit uses the microtiter plate format. Noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample. Sample Types: plasma, cell culture, tissue homogenates, biological fluids. Range: Dopamine: 0.3 - 100 ng/ml, Noradrenaline: 0.15 - 25 ng/mL. Sensitivity: Dopamine: 4.9 pg/ml, Noradrenaline: 2.4 pg/ml. Time: overnight ? The Dopamine & Noradrenaline High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples. The Dopamine & Noradrenaline High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures.