The Eagle Biosciences HDGF ELISA Assay Kit employs the sandwhich enzyme immunoassay technique for the detection of Human HDGF in serum, plasma and cell culture supernatant samples. An antibody specific HDGF has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any HDGF present is bound on the plate. After washing away an unbound substances, a Horseradish Peroxidase (HRP) conjugated primary antibody that binds to HDGF is added to each well and incubates. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of total HDGF bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450 nm ±2nm. The concentration of total HDGF in the sample is then determined by comparing the O.D. of samples to the standard curve. Sample Types: serum, plasma and cell culture supernatant. Range: 78 - 5000 pg/ml. Sensitivity: 30 pg/ml. Time: 3.5 hours ? HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. HDGF is a Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.