The Human High Sensitivity IFNγ (Interferon Gamma) ELISA Assay Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IFNγ has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNγ concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNγ Antigens and a biotinylated monoclonal antibody specific for IFNγ are successively incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 0.78-25 pg/ml. Sensitivity: 0.69 pg/ml. Time: 3h00 ? The Human High Sensitivity IFNγ (Interferon Gamma) ELISA Assay Kit is to be used for the in-vitro quantitative determination of interferon gamma (IFNγ) in human sera, plasmas, buffered solutions or cell culture media. The assay will recognize both natural and recombinant human IFNγ. The Eagle Biosciences Human High Sensitivity IFNγ (Interferon Gamma) ELISA Assay Kit is for Research Use Only.