The IL-2 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-2 Antigens and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 1.87-60 pg/ml. Sensitivity: 0.97 pg/ml. Time: 2h00 ? The Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in human sera, plasmas, buffered solutions or cell culture media. The assay will recognize both natural and recombinant human IL-2. The Eagle Biosciences Human High Sensitivity IL-2 ELISA Assay kit is for Research Use Only