The Eagle Biosciences anti-Pseudomonas aeruginosa IgG anti-Pseudomonas aeruginosa IgG EIA, E15, is a sandwich enzyme immunoassay. Serum or plasma samples are diluted and added to the wells of a microtiter plate, which have been previously coated with the Pseudomonas aeruginosa Antigenss alkaline protease, elastase or exotoxin A. Specific antibodies in the sample bind to the Antigenss present during an incubation of 2h at 37°C. After washing, the conjugate (anti-human IgG peroxidase-labelled immunoglobulin) is added and incubated again (for 2 h at 37°C). After a final washing step, substrate is added and further incubated for 30 min at room temperature. The reaction is terminated on addition of stop solution accompanied by a change from blue to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample. Sample Types: serum, plasma. Range: Cutoff. Sensitivity: 0.02 ng/ml. Time: 4.75 hours ? Pseudomonas aeruginosa, a Gram-negative bacterium ubiquitously distributed in the moist environment, causes about 10% of all nosocomial infections. This opportunistic pathogen leads to acute and chronic types of infection within the various organs. P. aeruginosa infection provokes a rapid production of antibodies to a large number of P. aeruginosa Antigenss. Eagle Biosciences's sensitive antibody detection system detects a P.aeruginosa infection very early at the onset. Due to the use of three P.aeruginosa Antigenss, which are highly immunogenic and present in different parts from nearly all P.aeruginosa strains. Depending on the Pseudomonas aeruginosa species and the immune reaction, antibodies can be detected against a single, two or even all three Antigenss simultaneously. A sample is regarded as sero-positive when antibodies against one or more of the Antigenss can be detected.