In the first step, ZnT8 Ab present in samples binds with ZnT8 immobilized onto the microtiter plate. In the second step, ZnT8-Biotin binds to this complex. The amount of ZnT8-Biotin bound correlates with the level of antibodies present in patient samples. Unbound ZnT8-Biotin is then removed by washing. Bound ZnT8-Biotin can then be quantified by addition of streptavidin peroxidase (SA-POD) and a colorigenic substrate (TMB), and reading the optical density at 450nm. Sample Types: serum, plasma. Range: 10 - 500 U/ml. Sensitivity: 10 U/ml. Time: overnight ? The Eagle Biosciences Anti-ZNT8 (Zinc Transporter 8) ELISA Assay kit is intended for the quantitative determination of Anti-ZNT8 (Zinc Transporter 8) in serum or plasma by enzyme linked immunoassay (ELISA). The Eagle Biosciences Anti-ZNT8 ELISA Assay kit is for research use only and not to be used in diagnostic procedures.