This Eagle Biosciences Bevacizumab ELISA Assay Kit is based on sandwich type ELISA. Diluted standards and samples (serum or plasma) are incubated in the microtiter plate coated with recombinant human vascular endothelial growth factor-A (rhVEGF-A). After incubation, the wells are washed. A horseradish peroxidase (HRP)conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Bevacizumab pre-captured by the rhVEGF-A on the surface of the wells. Following incubation, the wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Bevacizumab in the sample or standard. Results of samples can be determined by using the standard curve. Sample Types: serum, plasma. Range: 6 - 200 ng/mL. Sensitivity: 2 ng/mL. Time: 2 hours ? The drug Bevacizumab (trade name Avastin? ) is a recombinant human IgG1::k monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms and it has been approved by the FDA as a first-line treatment for metastatic colorectal cancer in combination with chemotherapy. Furthermore, VEGF is implicated in intraocular neovascularization associated with diabetic retinopathy and age-related macular degeneration. The specificity of this testsystem is achieved by using a monoclonal antibody named MAY-2B5 for the coating of the microtiter plate. This antibody is specific for Bevacizumab only and does not cross react with other VEGF catchers.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
anti-drug antibody kits, anti drug shikari shakari , Erbitux Ab, Antibodies, Antibody, ELISA Assay Kit EIA, Immunoassay, Cetuximab, IG-AB104, Tani