This Eagle Biosciences ELISA is based on Certolizumab pegol-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone CY). Standards and diluted samples are incubated in the microtiter plate coated with IG-CY mAb. After incubation, the wells are washed. A biotinylated recombinant human tumor necrosis factor alpha (rhTNFα) is added and binds to the Fab part of Certolizumab pegol. Following incubation, wells are washed and the horseradish peroxidase (HRP)-conjugated streptavidin is added and binds to the biotinylated rhTNFα. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Certolizumab pegol in the sample or standard. Results of samples can be determined by using the standard curve. Preincubation of Certolizumab pegol with rhTNFα inhibited the reaction in a concentration dependent manner. Therefore, the ImmunoGuide Certolizumab pegol ELISA (mAb-based) measures the free form of Certolizumab pegol. Sample Types: serum, plasma. Range: 6-200 ng/mL. Sensitivity: 2 ng/mL. Time: 3 hours ? Certolizumab pegol (trade name Cimzia?) is a tumor necrosis factor alpha (TNFα) blocker and binds to human TNFα with a KD of 90pM. Certolizumab pegol is a recombinant, humanized antibody Fab' fragment, with specificity for TNFα, conjugated to an approximately 40kDa polyethylene glycol (PEG2MAL40K). The Fab' fragment is manufactured in E. coli and is subsequently subjected to purification and conjugation to PEG2MAL40K, to generate Certolizumab pegol. Steady-state concentrations range from 0.5 to 90 μg/mL for a fixed dose of 400 mg of certolizumab pegol. The ImmunoGuide Certolizumab pegol ELISA (mAb-based) kit can be efficiently used for measuring free Certolizumab pegol levels in serum and plasma.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
anti-drug antibody kits, anti drug humira shikari shakari free ELISA Assay Kit EIA, Immunoassay, free Certolizumab Pegol, Cimzia,IG-AB109, AB109