The Eagle Biosciences Human Perforin ELISA Assay Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations and unknowns are pipetted into these wells. Sample Types: supernatant, buffered solutions, not suitable for use with serum and plasma. Range: 62.5-2000 pg/ml. Sensitivity: 40 pg/ml. Time: 2h45 ? The Eagle Biosciences Human Perforin ELISA Assay Kit is to be used for the in-vitro quantitative determination of Perforin in supernatant, buffered solutions. The assay will recognize natural Perforin.