The TRAIL Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations and unknowns are pipetted into these wells. During the first incubation, the TRAIL Antigens and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 93.75-3000 pg/ml. Sensitivity: 64 pg/ml. Time: 3h45 ? The Eagle Biosciences Human TRAIL/Apo2L ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of TRAIL in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human TRAIL. The Eagle Biosciences Human TRAIL/Apo2L ELISA Assay kit is for Research Use Only.