The determination of dog IgA is carried out as direct sandwich ELISA. An antibody specific for dog IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgA con cen tration. Sample Types: serum, biological fluids. Range: 15.625 - 1000 ng/ml. Sensitivity: 156 ng/ml (sample dilution 1:10). Time: 2.5 hours ? The Eagle Biosciences Canine (Dog) IgA ELISA Assay Kit is designed for the quantitative determination of canine (dog) IgA in complex samples (serum or other biological samples). The Dog IgA ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.