The determination of bovine IgG1 is carried out as direct sandwich ELISA. An antibody specific for bovine IgG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG1 present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG1 con cen tration. Sample Types: serum, biological fluids, milk, collustrum. Range: 7.8 ng/ml and 500 ng/ml. Sensitivity: 78 ng/ml (sample dilution 1:10). Time: 2.5 hours ? The Eagle Biosciences Bovine IgG1 ELISA Assay Kit is designed for the quantitative determination of Bovine IgG1 in complex samples (serum, milk, colostrum, or other biological samples). The Bovine IgG1 ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.