The kit uses a 96-well microtitre plate format. The serum specimen (diluted 1:400) is added to the antigen-coated wells and incubated for 30 minutes to allow food-specific antibodies to bind to the antigens in the microplate wells. After washing, the specifically bound antibodies are detected using goat anti-human IgG conjugated to horseradish peroxidase. After 30 minutes incubation, unbound conjugate is removed by washing, and TMB substrate is added. A blue colour develops in wells in which food-specific antibodies are present and this changes to yellow upon addition of Stop Solution. The absorbance of the yellow solution is then measured in a microplate reader using 450nm or 450/620nm filters. Sample Types: serum, plasma. Range: Negative <8 AU/ml, Borderline 8-12.5 AU/ml, Positive 12.5+. Sensitivity: Cut-off. Time: 1.25 hours ? Many people exhibit chronic food sensitivity reactions to specific food antigens. Unlike the immediate effects of IgE-mediated allergy, IgG-mediated food sensitivity reactions may take several days to appear. Controlled removal of the problem foods from the patient's diet will, in many cases, rapidly improve the patient's condition. General lethargy, weight gain, dermatitis, arthritis and tiredness are associated with food allergies. Irritable bowel syndrome may also be linked to food sensitivity.