The Eagle Biosciences Total Complement Functional Screen ELISA Assay Kit combines principles of the hemolytic assay for complement activation with the use of labeled antibodies specific for neoAntigens produced as a result of complement activation. The amount of neoAntigens generated is proportional to the functional activity of complement pathways. Sample Types: serum. Range: Cut-off. Sensitivity: Cut-off. Time: 2 hours ? The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the recently discovered MBL pathway.