The determination of the peroxides is performed by the reaction of a peroxidase with peroxides in the sample followed by the conversion of TMB to a colored product. After addition of a stop solution the samples are measured at 450 nm in a microtiter plate reader. The quantification is performed by the delivered calibrator. Sample Types: serum, plasma, cell culture. Sensitivity: 6 μmol/l. Time: 15 minutes ? In a healthy body oxidative and reductive processes are in a balance. Free radicals (reactive oxygen species) are eliminated by antioxidants. In case of a lack of antioxidants, free radicals react with cell structures. A reaction with unsaturated fatty acid leads to lipid peroxidation products. These circumstances are under discussion during the genesis of atherosclerosis. Recent findings suggest that free radicals are also involved in inflammation, sepsis, carcinogenesis and neurodegenerative diseases. An increased level of peroxides in blood indicates oxidative stress in the body. The measurement of peroxides can indicate an increased risk for heart attack and stroke. A subsequent change in nutrition and/or supplementation therapy might reduce the risk. The Total Lipid Hydroperoxide ELISA kit allows an easy, rapid and precise quantitative determination of the total peroxides in biological samples. The kit includes all reagents ready to use for preparation of the samples.