This Urinary Isoprotane ELISA kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t-HRP bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards. Sample Types: urine. Range: 0.05 - 100 ng/mL. Sensitivity: 0.05 ng/mL. Time: 2.5 hours ? Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 15-isoprostane F2t (also known as 8-epi-PGF2a or 8-iso-PGF2a) in urine samples. Levels of 15-isoprostane F2t in urine are useful for the non-invasive assessment of oxidant stress in vivo. 15-isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis.