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Description
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IκBα (Tyr-305) peptide (without carrier). The antibody detects a 38 kDa protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but does not detect this band in control cells.
Immunogen
IκBα (Tyr-305) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 305 of human IκBα. This peptide sequence has low homology to other IκB proteins.