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Description
This antibody was affinity purified with phospho-VEGFR-2 (Tyr-801) peptide. The purified antibody detects a 180 kDa band corresponding to VEGFR-2 in western blots of human endothelial cells treated with pervanadate, and shows strong reactivity toward recombinant human VEGFR-2. This reactivity is removed by akaline phosphatase treatment. In addition, the antibody detects the conserved site in VEGFR-1 (Tyr-794). The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.
Immunogen
Phospho-VEGFR-2 (Tyr-801) peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-801 in human VEGFR-2. This sequence has high homology to the conserved site in rat and mouse VEGFR-2, and has significant homology to the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812).