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Description
This antibody was affinity purified with VEGFR-2 (a.a. 1304-1317) peptide. The purified antibody detects a 180 kDa band corresponding to VEGFR-2 in western blots of human endothelial cells, and shows strong reactivity toward recombinant human VEGFR-2, but not VEGFR-1 or VEGFR-3. The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.
Immunogen
A synthetic peptide (coupled to carrier protein) corresponding to amino acids 1304 to 1317 in human VEGFR-2. This sequence has high homology to rat and mouse VEGFR-2, and no significant homology to other VEGFR family members.