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Description
This antibody was affinity purified using phospho-SK2 (Thr-578) peptide (without carrier). The antibody detects 70 kDa proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Immunogen
Phospho-SK2 (Thr-578) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-578 in human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and is not well conserved in SK1.