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Description
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1513) peptide then affinity purified using phospho-TRPM7 (Ser-1513) peptide. This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 and rat PC12 cells stimulated with calyculin A, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Immunogen
Phospho-TRPM7 (Ser-1513) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.