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Description
This antibody was cross-adsorbed to phospho-βIII-Tubulin (Ser-444) peptide before affinity purification using unphosphorylated βIII-Tubulin (Ser-444) peptide (without carrier). The antibody detects a 50 kDa protein corresponding to the molecular mass of unphosphorylated βIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.
Immunogen
Unphosphorylated βIII-Tubulin (Ser-444) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 444 of human βIII-Tubulin. This sequence is not found in bI or βII-Tubulin isotypes, but is well conserved in βIII-Tubulins from rat and mouse.