Acetobacter Agar (Mannitol) is used as a maintenance medium for mannitol positive bacteria. Acetic acid bacteria are found in fruits with high carbohydrate concentration, which is selective for yeasts, that produce ethanol. This ethanol forms the substrate for acetic acid bacteria and may oxidize ethanol to acetic acid (1). Various synthetic and maintenance media for Acetobacter cultures have been reported (2). A typical maintenance medium is Acetobacter Agar (2) which has been formulated as per Manual of Microbiological Methods (3) and being used for the maintenance of Acetobacter species utilizing mannitol (4). Peptic digest of animal tissue, yeast extract in the medium provides nitrogen, vitamins and minerals necessary to support bac terial growth. Mannitol acts as energy source. Calcium carbonate acts as a buffer.