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Description
Clone M033 was purified using Protein G chromatography. The antibody detects 75-100 kDa bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of native A431, HeLa, MCF7, and MDA-MB-231 cell lysates, but does not detect the denatured form of CD55. The antibody also detects a native human recombinant CD55 protein that includes the extracellular region. The antibody can be used for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry, as well for detecting CD55 in live, unfixed cells.
Immunogen
Clone M033 was generated from a proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.