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Description
This antibody was affinity purified using phosho-Coronin-1B (Ser-2) peptide (without carrier). The antibody detects a 60 kDa band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.
Immunogen
Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.