If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
This antibody was cross-adsorbed to phospho-C-Raf (Ser-338) peptide before affinity purification using phospho-C-Raf (Ser-471) peptide. The purified antibody detects a band at 74 kDa corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).