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Description
This antibody was affinity purified using phospho-CrkL (Tyr-207) peptide (without carrier). The antibody detects a 38 kDa protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
Immunogen
Phospho-Crk (Tyr-207) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-207 in human CrkL. This peptide sequence is well conserved in mouse and rat CrkL, and has 40% homology with the conserved site in Crk II (Tyr-221). The site is not found in Crk I.