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Description
The antibody was cross-absorbed to unphosphorylated eEF2K (Ser-445) before affinity purification using phospho-eEF2K (Ser-445) peptide (without carrier). This antibody detects a 105 kDa protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.
Immunogen
Phospho-eEF2K (Ser-445) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-445 in human eEF2K. This sequence is well conserved in rat, mouse, and fish eEF2K, and has low homology to other proteins.