The determination of chicken IgM is carried out as direct sandwich ELISA. An antibody specific for chicken IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM con cen tration. Sample Types: serum, biological fluids. Range: 3.9 - 250 ng/ml. Sensitivity: 98 ng/ml (sample dilution 1:25). Time: 2.5 hours ? The Eagle Biosciences Chicken IgM ELISA Assay Kit is designed for the quantitative determination of chicken IgM in complex samples (serum or other biological samples). The Chicken IgM ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.