Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilize proteins, glycoproteins, or nucleic acids. Boster's Nitrocellulose Membrane, 0.25 μm, 9 cm x 10cm is used for low molecular-weight proteins and nucleic acids <500 bp.