The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human Interleukin-8 (IL-8). FluoroSpotBASIC (550) contains monoclonal capture and detection antibodies, a secondary fluorophore-conjugated detection reagent (SA-550) and a Fluorescence enhancer. Cross-react with IL-8 from non-human primates (NHP), cow and dog..