The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human TNF-alpha (TNF-α). FluoroSpotBASIC (550) contains monoclonal capture and detection antibodies, a secondary fluorophore-conjugated detection reagent (SA-550) and a Fluorescence enhancer. Cross-react with TNF-α from non-human primates.