The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The monkey IFN-γ/IL-2 FluoroSpot kit is perfect for users who wish to have a The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The monkey IFN-γ/IL-2 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. The included anti-CD3 mAb can be used to induce cytokine secretion and is dependent on number, nature and viability of accessory cells. The antibody cross-reacts with CD3 on cynomolgus and rhesus macaque T cells. React with human IFN-γ and cross-react with IFN-γ from non-human primates.