Human AFP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AFP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: R19-V609) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and