Purity ≥98% by SDS-PAGE gel and HPLC analyses. HCC-1 is a CC chemokine that signals through the CCR1 receptor and chemoattracts blood monocytes. It is secreted by various tissues including skeletal muscle, heart, spleen, liver, bone marrow and plasma. Mature HCC-1 is found in four different forms, which are distinguished by differential N-terminal truncation and contain 74, 72, 71, or 66aa residues. Recombinant human HCC-1 (66aa) is a 7.8kD protein consisting of 66aa including the four highly conserved residues present in CC chemokines. Biological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 5-20ng/ml. AA Sequence: GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN Quality Control: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Endotoxin: <0.1ng/ug of protein (<1EU/ug). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.